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NEET Biology: Photorespiration C3 and C4 pathways Practice Questions

13 free multiple-choice questions on Photorespiration C3 and C4 pathways (Photosynthesis and Respiration) for NEET Biology, each with the correct answer and a step-by-step explanation. Attempt a question, then reveal the solution to check your reasoning.

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  1. Q1. Photorespiration is an energy-wasting process that occurs when the enzyme Rubisco binds to which molecule in the presence of low CO₂ concentrations?

    • ACO₂
    • BH₂O
    • CO₂
    • DRuBP
    Show answer & explanation

    Correct answer: C — O₂

    Photorespiration occurs when Rubisco, instead of binding CO₂, binds O₂ on the same active site, leading to the emission of O₂ instead of CO₂.

  2. Q2. The C₄ pathway effectively concentrates CO₂ around which specific enzyme to maintain efficient carbon assimilation?

    • ACytochrome oxidase
    • BPEP carboxylase
    • CRuBisCO
    • DATP synthase
    Show answer & explanation

    Correct answer: C — RuBisCO

    In C₄ plants, CO₂ is initially fixed by PEP carboxylase and then transported to the bundle sheath cells where CO₂ is concentrated around RuBisCO, reducing photorespiration.

  3. Q3. The C4 pathway involves the initial fixation of CO₂ by which initial enzyme in the mesophyll cells?

    • APhosphoenolpyruvate carboxylase
    • BATP synthase
    • CRubisco
    • DPEP carboxylase
    Show answer & explanation

    Correct answer: A — Phosphoenolpyruvate carboxylase

    In C4 plants, PEP carboxylase fixes CO₂ into a four-carbon compound (oxaloacetate), which is then transported to the bundle sheath cells for the Calvin cycle.

  4. Q4. In C4 plants, the initial CO₂ fixation occurs in the mesophyll cells. The resulting four-carbon compound is transported to the bundle sheath cells where it is released. Which compound is typically used for transport in the C4 cycle?

    • ARuBP
    • BO₂
    • CMalate
    • DCO₂
    Show answer & explanation

    Correct answer: C — Malate

    Malate (or aspartate) is the primary organic acid used for the transport of the fixed carbon from the mesophyll cells to the bundle sheath cells in C4 plants.

  5. Q5. Which of the following is a primary consequence (loss) of photorespiration in photosynthetic organisms?

    • AIncreased utilization of light energy
    • BIncreased efficiency of ATP production
    • CRelease of CO₂
    • DNet production of glucose
    Show answer & explanation

    Correct answer: C — Release of CO₂

    Photorespiration results in the wasteful consumption of energy and the release of previously fixed carbon as CO₂, reducing the net production of sugars.

  6. Q6. Which group of plants utilizes the C4 pathway?

    • AWheat and Rice
    • BSpinach and Tomato
    • CMaize (Corn) and Sugarcane
    • DPeas and Beans
    Show answer & explanation

    Correct answer: C — Maize (Corn) and Sugarcane

    C4 plants are commonly found in crops like maize and sugarcane, which utilize the C4 mechanism for efficient CO₂ assimilation.

  7. Q7. The enzyme RuBisCO primarily catalyzes which reaction in photosynthesis?

    • AReduction of CO₂
    • BSplitting of water molecules
    • CFixation of CO₂
    • DPhoto-oxidation of water
    Show answer & explanation

    Correct answer: C — Fixation of CO₂

    RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase) is responsible for the initial fixation of atmospheric carbon dioxide into organic molecules.

  8. Q8. In C4 plants, the initial fixation of CO₂ occurs in which specific cell type?

    • ABundle sheath cells
    • BCytoplasm
    • CMesophyll cells
    • DChloroplasts
    Show answer & explanation

    Correct answer: C — Mesophyll cells

    C4 plants utilize a spatial separation mechanism. Initial CO₂ fixation occurs in the mesophyll cells by PEP carboxylase, fixing CO₂ into a C4 acid. This acid is then transported to the bundle sheath cells where the CO₂ is released and fed into the Calvin cycle.

  9. Q9. The primary drawback of the C₃ pathway compared to the C₄ pathway under standard conditions is:

    • ALower rate of ATP production.
    • BHigher occurrence of photorespiration.
    • CIncreased water loss.
    • DInability to produce glucose.
    Show answer & explanation

    Correct answer: B — Higher occurrence of photorespiration.

    C₃ plants suffer significantly from photorespiration because RuBisCO can bind O₂ instead of CO₂, leading to the wasteful process of photorespiration.

  10. Q10. In C₃ plants, what is the main biochemical limitation that occurs when the oxygenase activity of RuBisCO is favored over carboxylase activity?

    • APhotooxidation
    • BGlycolysis
    • CExcess ATP production
    • DPhotorespiration
    Show answer & explanation

    Correct answer: D — Photorespiration

    When O₂ binds to RuBisCO instead of CO₂, it leads to photorespiration, a wasteful process where fixed carbon is released, reducing photosynthetic efficiency.

  11. Q11. In the C3 pathway of photosynthesis, the primary initial fixation step involves the reaction between CO₂ and which organic molecule?

    • A3-PGA
    • BO₂
    • CRuBP
    • DPEP
    Show answer & explanation

    Correct answer: C — RuBP

    The initial fixation step in C3 photosynthesis is the carboxylation reaction where CO₂ is fixed onto Ribulose-1,5-bisphosphate (RuBP), catalyzed by Rubisco.

  12. Q12. What is the function of the CO₂-concentrating mechanism employed by C4 plants?

    • ATo enhance the activity of PEP carboxylase
    • BTo increase the rate of water evaporation
    • CTo reduce the photorespiration by concentrating CO₂ around Rubisco
    • DTo increase the overall rate of the Calvin cycle
    Show answer & explanation

    Correct answer: C — To reduce the photorespiration by concentrating CO₂ around Rubisco

    The CO₂-concentrating mechanism ensures a high local concentration of CO₂ within the bundle sheath cells, minimizing the opportunity for Rubisco to react with O₂ and thus suppressing photorespiration.

  13. Q13. What is the primary biochemical disadvantage of photorespiration in C3 plants?

    • AIncreased O₂ fixation in the Calvin cycle
    • BIncreased consumption of ATP and release of fixed carbon
    • CReduced overall ATP production
    • DLoss of fixed carbon
    Show answer & explanation

    Correct answer: A — Increased O₂ fixation in the Calvin cycle

    Photorespiration results in the wasteful consumption of ATP and the release of previously fixed carbon, making it an energy-loss process for the plant.

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